FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_pIXHALO_col_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_pIXHALO_col_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665609
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG4500.016.3319470
CAACCCG4751.2732926E-1113.262423539
CGGCGTG2601.1775897E-412.11471461
GCCTAAG11000.011.45425555
AACCGCA12800.011.21031834
CTAGCTG3155.6453035E-511.112164521
CCCGATC6006.366463E-1111.08272142
GTAGGGG4451.3972203E-711.01029570
CCTTGTT14800.010.8774570
GCGTGTT2300.007896204510.65126570
CCTAAGT11250.010.57751156
GCGGCGT2650.001871446410.56545460
GAACTTA15300.010.52229427
TCGTGTG4702.9676085E-710.42464270
CGCATTC2700.002182962810.37166519
AAACCGC12600.010.27720933
ACCGCAA12050.010.16541335
TCCTTGT17000.09.8815169
TCGGTGG3208.0474885E-49.8429170
CGCAACC9700.09.74172937
Produced by FastQC (version 0.11.5)