FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n02_coldna_daplib_dapv1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_coldna_daplib_dapv1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAGGTTGGTTAGTGTTTTGGAGTCTACTATTACATTATATCATGTTTA12.272727272727273No Hit
CTTTCATGGTGTAGGCAAAGACCATATGAGTGTTTGGCTTTGTGTCTTCT12.272727272727273No Hit
AACTGTTGCTCGTAAGATCGCTTTCCCCAAAACTGAAGTTGTGTCATTAA12.272727272727273No Hit
AAAGGTCTAAACAGTATATGCTCTGTTTAGCCGTTGCAACAAACTTCATC12.272727272727273No Hit
TAGTTTCATTAGCTCGAATATAGCAGTAAGTGAATACGATATAAGACAAC12.272727272727273No Hit
GTACTTGCCTCCATCAGTAGCCCATTTGGATCCATCCCAAATGGTTGAAT12.272727272727273No Hit
CGAACATTAATCATGCTTATCAAAGCTTTCAATGTGTATCCAAAATACAT12.272727272727273No Hit
GGGTAGTGTTGGGAGGAGATTCGGGGATCGGGTTGGTATTTCTCATATTA12.272727272727273No Hit
AAAAGATGGCAACATCACGGCTAAAGCTTTGTTAGAGACGTTTATGCTCT12.272727272727273No Hit
CCCTCCAGTGAACAACTCATCTATCTCCTCGCATAGCTCCCAGTACTTCC12.272727272727273No Hit
ATAGATGTATGCGAAACTCCATCGCCACCATAAATAGGCCTACCTCCCTG12.272727272727273No Hit
ATAAAACCTAGTGTTACAGTTTTATTATTAAATAAGCTAAATTATAAAAA12.272727272727273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGTGGAG12.272727272727273No Hit
CTGGAAGTAGTCAGAAGATATCAAGTTTAGTTATCTGGCGACTCTTTAAC12.272727272727273No Hit
AACATACACATGACATCAAGTCATATTCGACTCGAAAACACTCACCAAAC12.272727272727273No Hit
AAAAGGCTTGAGGGGGAGCATATCTCCTGATCTTGATTCATAGTTACATA12.272727272727273No Hit
GGCTTTGAGTTTGGGTTGCTCATGGCAACAAAGATGAGGATTCTACGTCC12.272727272727273No Hit
ATACTCAATCATACACATGCCATCAAGTCAGATTCGCCTCCAAACCACTA12.272727272727273No Hit
TGTCCATATGAGAATCAAGCGTCGAAGGAACCCAGTCGAAACCCTACAGA12.272727272727273No Hit
GAGAACGGGACGGGACAAAATCTGAGGCAATTATTGTTGTGTGCAGAGAA12.272727272727273No Hit
ATCAATGATTTCCACATATGGCGGTAAAAAAATCTCAACAGCACTAATAT12.272727272727273No Hit
AATAGGAAATGCAAGGACCATCGAGAAGATTCACAGAAGAATGTTGAATT12.272727272727273No Hit
ATAGCTGAAGTGAATACCATGCAAGTTTAGTTGCTCAATGAGTCTTTTGA12.272727272727273No Hit
CTTTGCCTACACCATGAAAGCTTTGAGAAGCAAGAAGAATGTTGGTTAGT12.272727272727273No Hit
CAGACATGATAATTAAAGATCATATTCAGGTTCAAATTGACAGGGAATGG12.272727272727273No Hit
GTATGGTGGAGTAGGTTTTATTCCTTATCTATAGATTAGAATCGCAACCC12.272727272727273No Hit
ATGGCAATATCCCTACGGATATATAGATAATCATGGGTTCCCTCCAAACT12.272727272727273No Hit
CACTTGACTGAACTGTTAACAAAGTATACGTAATTGAAGCTGGGGCTGGA12.272727272727273No Hit
GACATAGAGATCGTTGAAACTACAACAGGATTAAGTTCAAGCACTAGACA12.272727272727273No Hit
GTGTATGATTGAGTATAACAACTTAAACCGCAACCCGATCTTGTAAGCCT12.272727272727273No Hit
CAATATGACTTGATGTCATGTGTATGATTGAGTCTAACACCTTAAACTGC12.272727272727273No Hit
AGCTATCAACTTGACTAAGACGTTCCACGAAAAGGCGCAGAGATGCGAAT12.272727272727273No Hit
CAATGCACTTAGCAAAGCTAATCTATTCGTTCTCATTTAGGCAGAAATGT12.272727272727273No Hit
TAGAAGACACAAATCCAAAGACTCATATGGACTTTGGCTACACCATGAAA12.272727272727273No Hit
TATTTTTTGTAATTCCACCCGGTTATCAAGCACATTTTTAGTCGACTAAT12.272727272727273No Hit
GCGCTCTATCATCAAATTCTTAGGGTTCTAGAATCCACCACTCCCCTCTC12.272727272727273No Hit
TACAAAAACCTGAGTACCGAGTGACGCTCACTCGCGGTCGTAAGAAGATA12.272727272727273No Hit
GTTTCCTTGTTTGAAGACATAAAGCCAAAGACTCATATGGACTTTGGCTA12.272727272727273No Hit
CTTTGGAGTTCGTTTACACTCCACATCCATTTTTTCTTGCCTTGACTTAG12.272727272727273No Hit
AGCAAAACTCCATATGCTGCGGTTGATTTTAGACTTATAACAAACAAAGA12.272727272727273No Hit
AAACAGCAACCATCTTCTTTCATCGTAACTAGTTAATCCTATATCCAACG12.272727272727273No Hit
TTGGAAGATACCAACCTCTTGCCTTCGCGACTCATTTCTGAAGCGACCTG12.272727272727273No Hit
CACAACGAGATAAAAAAACCAACAGCACACCAAGCAAAAACAATAACTAT12.272727272727273No Hit
CATGAGGTTTGCAGGTGTTCTCGTGCAACTATTTTTGTAAGTCCAACAAT12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)