FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_coldna_daplib_dapv1-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_coldna_daplib_dapv1-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413519
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52760.3732528533397853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG4250.026.34969170
CGGTGGG3800.022.1025470
GCGTCGG7250.018.82526470
GGTCGCC3704.3655746E-1115.13326870
GAGTGTT16100.014.78078670
GTTCCCC1500.00593262514.00124612
CTCGGTG9250.013.99827470
AAAGAGG12900.013.56421970
GTAGGGC4701.0913936E-1113.40260370
GGAAAGG4204.6820787E-912.49845970
TCGGTGG9800.012.1413669
GTAGGGG12000.011.95685970
TGTCGGG2050.003350160611.94974670
AGAGCGG10950.011.82502670
GTAGATG11450.011.61428870
TCGGGGG3953.5693665E-711.51756769
CTGAGCA2150.004778011211.39515327
GGAAGAC6251.0913936E-1111.19861970
TCTCGGT11700.011.06701169
TGGTCGC4752.8947397E-811.05126969
Produced by FastQC (version 0.11.5)