FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_c24dna_daplib_dapv1-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_c24dna_daplib_dapv1-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983300
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48300.4912030916302247No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA950.007211966418.4201344
GCGTCGG2851.2732926E-1118.41825770
GTAGGGG4051.0913936E-1114.68912770
GTGGTCG3456.1229E-813.18640370
GGTGGTC3254.2407555E-712.92111469
ATCTCGG4601.8011633E-811.41131170
AAAGAGG5251.1004886E-810.66504870
TCGGAAC2750.002546554210.18027270
CGGTGGT3451.4225264E-410.14338768
AGAGGGT3451.4225264E-410.14338768
AGAGCGG3551.8978817E-49.85765870
CTCGGTG2900.0039529719.65370766
GATCTCG4602.7177924E-59.12904969
TCGGTGG3854.285099E-49.08952967
GGGAAAA8051.4078978E-98.69433270
AGATCTC6704.7125468E-78.3569470
AGAGTGT22300.08.31712270
AGACTTA3800.00383367558.2907442
GAAGAGG5653.4117744E-58.05187570
AGGGGAA4350.00144172118.04475564
Produced by FastQC (version 0.11.5)