FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_abdna_ablib_dapv1-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_abdna_ablib_dapv1-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401530
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26800.19121959572752634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG5250.021.99701770
CGCCCTA1003.937827E-420.99715468
TCGCGGG1356.0332877E-620.7379370
GTCGCCC2254.638423E-918.66413770
TCGCCGG3005.5479177E-1016.3311270
TCTCGGG5250.015.99783170
GGTGGCC4050.015.55344770
CGCCGTA3151.1095835E-915.55344770
AAGGGGG2755.520633E-815.27065766
GGTCGGC3009.760697E-915.16461170
TAGCCGG1400.003757855914.99796769
TCGGCGG1400.003757855914.99796770
CGTGGGG1450.004744860314.48079569
GCCGTAT3454.0090526E-914.20097369
AGGTGGG2759.178002E-713.99810170
GTCGGCG1750.001019185513.99810169
GGTCGCC9400.013.77472970
GCGTCGG2801.1244829E-613.748136570
TCGTGGG2052.1653685E-413.65668668
GTAGGGG6500.012.92132570
Produced by FastQC (version 0.11.5)