FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n02_WRKY40_col_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_WRKY40_col_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAACCACTGGTCGACAAATCCTCGGCCCCGGTCGCCGCTTACTCGGCC14.0No Hit
TTACAGAACCTTTTTCTCAGCTTAAGAAATATGTACACTTTTCAAAATTC14.0No Hit
GTATAAGACCTTAAACCGCAACCCGATCTTAAAGGCGTAAGAATTGTCTC14.0No Hit
ACATCATCAGCTCGTCAATTCCTGGGATTCTTTCAACATGGTGCAAGGTA14.0No Hit
CAATGATGAGCCAAAGTGGCATAAGCACTCGATAGCACATACTAACCATA14.0No Hit
ATGTTAGATCCTTGACTTCCTGTCAGCGCCAATATTTTATTATAATATTA14.0No Hit
ATCGTATAGATTTTCTGATAGGCACAAGAAATGATTCATGCGAAAGATCC14.0No Hit
CGACACGCTTAGGTCATCACTATCTAGGCTCCGTTTGATTGGTTCGGTCG14.0No Hit
GATTTCTCGGTCCCGGTCGACCATTGCCCCTCCGCGTCGACAACACCTCG14.0No Hit
AAGTGAAGTCATTGCTTTGATAGACGTTGACTGTTCACAAAAACACCTAG14.0No Hit
TAAAGGATTTAAGTAAACAAATTGTAGTTTGCAAGTGAAGTTTTAAGGGA14.0No Hit
AGTATTAGAAGTCCGCTCGATTGATTCAATATCGTTGAACTTAGAAAAGC14.0No Hit
AGAAATAGTGTGAGAGATCAAGAATGCTGACTATTTCTTAGATTCATGGA14.0No Hit
GACCTCAAGTAATATTCGACTCCAAAACACTAACCAACCTACTTCTTGGT14.0No Hit
ATTAATTACATTTTGACCGAATTGACCAAAAACTTTGTGATAGGAATCTT14.0No Hit
AAGCGAAGACTCGCTACGTTTGAATATCTAGAATTATGTTTGTCGTACAC14.0No Hit
TTAGGATGATTAGTATCGCAAAACACATATTTCAGTGTGTAAATACCCCA14.0No Hit
TAATATGAGGGTGGTCCTGTTAGGCTGATAATGGTCTGGTCCAACTCCTC14.0No Hit
GAAAGTCGTGAATTTCTAATCGAGTTGAATGATATAGGGCCGGTGAAATA14.0No Hit
CCTTTATAACGCTACCCCACCTTCGTATTGATTCTCAACGCTTTCTTTGT14.0No Hit
GCAAGAAAGACGCACATCAATCAGAAACGGGAAGGTTGTGGTTAAGGGAT14.0No Hit
GCAAGCTGCGAACCTTGCTTAACACCACAATTCTCAATTAATAATTGAAT14.0No Hit
ATGTAATCTAGCAAAGAAAAGTCCTAAACTGTATGGTACAAAGTCATATT14.0No Hit
GTGGCTAAAAGACTAACGAACCTTCTTATTGATGTTCAAAGCTTTCATGG14.0No Hit
AACCAGATCTTGTAAGCCTAAGTAGTGTATCCTTGTTAGAAGACACAAAG14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)