FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n02_WRKY40_c24_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_WRKY40_c24_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATGAGGTCAAGACAGAAACTTAAGTCTACTAAAAAGTGCAAAAGAA11.4084507042253522No Hit
TAAGTATTCGCGCATCTTTTTTTCTTCGCTTTAGCCTTTCTAGTCAAAGA11.4084507042253522No Hit
GTGTGAGGAATAAGAGTCAGATAGAAGTCCTTACGACATTCCTATGATAA11.4084507042253522No Hit
CACCAATCTTATATGACATTTTAAAGATATTTTAAAGCGAGGATTTTGAC11.4084507042253522No Hit
AAGAGAAGAAGTCATATACGACTCCAAAACACTTCCCCACCTAATTATTG11.4084507042253522No Hit
CAGAAACCAGCTATCATTTAGAAGGTCAATTAGAATCGGGCGGGGGATTG11.4084507042253522No Hit
CACGATCTAAATCGTTCTGTGAACGATGATATTGACCGGTTCATAAGATG11.4084507042253522No Hit
ATTCGCTGTTAACTTCTGCTCCTCTAAATTGTAGTCAAGCCAATCAACAA11.4084507042253522No Hit
AAGTTGCTTCATTCATGGAGCCAAGTTAGAAGTCAATTGCAGCTGGGCGG11.4084507042253522No Hit
AAATGAAGAAACAAAAAAAGAAAGTGAAAAGCATTATTTGGAGCTTTTGC11.4084507042253522No Hit
GGTCGACGATTTCCACTCCAGGTAGAAAAATACTCGGACCCCGAGGATGT11.4084507042253522No Hit
ATTACCCACAACCACCGCAGGAAACCCGTTCTCGTGATCACGTCATGCAT11.4084507042253522No Hit
AAATCGTCGACCAGGTCCGAGACTTCATCGACCGGGTCCGAGGAATCGTC11.4084507042253522No Hit
ATTATTACTCAGACTGCACCAATTATCATTTACTTTTTATTCAAACTCCT11.4084507042253522No Hit
CCACCAACGTGACAGAGAATTCCAGGGTAGTTACCGGAGCTCTACTGGTC11.4084507042253522No Hit
ATTTAACTTTGAATTATACTTATATATGCTCATACATAAATAAAACACTA11.4084507042253522No Hit
GGATACTAAGTAGCAGTCAAAAATTTGTATCCATTTTTCATGATATTATG11.4084507042253522No Hit
AATTGGCAACGTTGAGGTTGTCTCTGATTAAACCGGTTATGATCATGTAA11.4084507042253522No Hit
ATGACATTTAGAAAAATTAGACACCAGAAACCGATCCTCCGGTCTGCAAA11.4084507042253522No Hit
CCCTCTGAACCGCCCGAATCGGGACGTGAGAGAGCTTCTGAGTAAGTGCC11.4084507042253522No Hit
ATCTCAAATCAGCCAACTTAATTTTATTAGTCTTATCTCAAAGTTGTGTG11.4084507042253522No Hit
CTGGTGTAGCCAAATTCAACAGCAGCCGATGCCTATGAGTCATATAAAAA11.4084507042253522No Hit
TTCCCGGTGCTTTGTTTTTAAACGAATAATCCTTAGACATTGCCAAGAGC11.4084507042253522No Hit
CTAGTACTTATCGTAAGCTGTTCTCGCTTTAGAGTCTTGACTATGTTATA11.4084507042253522No Hit
CAAAAAGGCAACCTTAGACAAAGGACAATGGACAGCTGAAGAAGACACGT11.4084507042253522No Hit
AAAATTATTCTTTAATATACATGCTTACAAGGTTTAGCCAAAATCAATAT11.4084507042253522No Hit
ATGACCATGAACCATTGTTTCCTCCAGGTGTGGTATTCTCTCGTGAACAA11.4084507042253522No Hit
ACACTGCTTCAATACTAACGGAATGGTTGTTTGTAATTGTTTGATTGATG11.4084507042253522No Hit
GGGTTTGAATCTACGAGAAATTCTAGAGAATGTTTGCTACCCTGAAATTT11.4084507042253522No Hit
TACTTTTATTGAGATAACGCGTTGAAAGCTTACCAAATTTTGGTGGATTA11.4084507042253522No Hit
CTTTCCTTCCGTGCCGTAGGTCAATGAAATTATCTTTTTCAGCACGAGAA11.4084507042253522No Hit
TGATCAAATAACCCGGAAATTCGAATTGCTTAAGACAATAGAGGAAGGAC11.4084507042253522No Hit
ATAACTTGAGCATTAAGAGAATAATTGTTCATCCCCCTTAATCAAATAAA11.4084507042253522No Hit
AATTCGATTGGGTAAGTGACTTGTTCTTTATATTATTTTGATAATAACCG11.4084507042253522No Hit
CATTTCAGAACTCAAGAAAATACAGACGTTCCCTTCAGTTCAAATCGCAT11.4084507042253522No Hit
ACTTGACTTTACCAACTCCTCTAACTTCAGAGAAAGAGTTGTTTGCCATT11.4084507042253522No Hit
TGTTTTTGGAAAAGAGTCCAATGGTCCCCGCTGGTGGAGTTATGGTTGAG11.4084507042253522No Hit
CCACCCGGACGATCGAGTAGAGGAGCTGGGCGACCCATTCCAAGGCTTCT11.4084507042253522No Hit
AGAACCATCTCTACTTGATCTTCAAAACAAATTCGGCGCGAGAACAGATC11.4084507042253522No Hit
CTCAATAATAATATCGTTTACCATTACAAACTACAACTTAAACCGCAGCC11.4084507042253522No Hit
CAACGGTCAAGATTTCGTCGTCCAGATAGAGCAGCGATACGTCACGCCAT11.4084507042253522No Hit
TTTTTAAAGTGTTCTTGACTTGAATTGACTTCAGGTTGTTATGTCGTTTC11.4084507042253522No Hit
ATCGTTACATCAATAAAAATTCAATTGAAGAGAAGAAACTATTAGTTTCT11.4084507042253522No Hit
ATTAAACTGCTTAGATTATAAAAAAGGGAACACGAGAACGGGATGAGGGT11.4084507042253522No Hit
TCATGCTTCAATATCTTTTATTTTGAATAACATCAAACAAACTAAAACTA11.4084507042253522No Hit
GGAATATTCCTATGTTATATTAAATTTACCATATTCTAACACTGAAAGAC11.4084507042253522No Hit
AGATGAATGAAGAATAAATGATAGTGTTTTCCAGTCTAATATACCGAGTT11.4084507042253522No Hit
GGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCG11.4084507042253522No Hit
CAAGAACGGAGTCCTCTTTATAACTATCCCTAACACCAAAATCCAACGCC11.4084507042253522No Hit
TATGCATAAACATATATACCATCCGAGGTAATGCAATTTAGACAAAAACC11.4084507042253522No Hit
TATCATTTGTTTGCCAGCGTAAACTAGGCTTCATTATAGATATGTTTTCG11.4084507042253522No Hit
ATACTCCATACGCTCGGCGTCTGGAAAGATCTTCTGTTGAAGAAAGTCTC11.4084507042253522No Hit
ACAACCAATAACGACACCAAGAGTTTCCTGGTAGTAAAGTGCTGAATCGC11.4084507042253522No Hit
CAGTTGCACTCGGCACGTCTTTTGCGTAAAGAGATGAGTAATAGATAACG11.4084507042253522No Hit
CTGACCAGAGAGTGAGGAGGGTAGACGAACGACAAGAAGCCACGAAAATG11.4084507042253522No Hit
AGAGAGTGTGCTAGTGTGTAATCTTGACTCAACAATTTCCTGGTCAAAGT11.4084507042253522No Hit
AATATAACGGAAGAGAATATAGATAGATTACCATTATACATGACAATACT11.4084507042253522No Hit
AACCATTCTGCGGTTAGGTCCAAACATCAAACAACCAAGCCTGAGGAAGA11.4084507042253522No Hit
AAAGATAGAATGACAGTAAGCAACGTATTTAAATGAAAATGGGAGATGGC11.4084507042253522No Hit
AATGTATTAGACGAAGATCATATTTAGCATGGAGTAATAAACGTTATGAA11.4084507042253522No Hit
AAGTGAGTTGTATATTTATTACTTTATTTCTTTGATAAATTTATGTATAT11.4084507042253522No Hit
CCTAATCCATCTCTATCTCTAATAAATAGTCAAATTTAATGGATTATGAT11.4084507042253522No Hit
ATTCTTCTGTTTTCCACGTGCCCATCTTCAGAATTATTTTCGAAGAGGGA11.4084507042253522No Hit
AGCAAACACTACATAGACTTATAAGATCCGGTTCCGGAATAAGTACTTAT11.4084507042253522No Hit
GCGGTTGGTGAGAGGTCTCGCGTCGAATCGGTCATGATGGAGTGAGATGC11.4084507042253522No Hit
ATTTGAGTTATAATAGTTTTTGACTAAGATTGGGAGTGATTTATGCTAAA11.4084507042253522No Hit
GAGCTGCATGGGCCTCTGCTCAGCCGACGAACTGTATCCGGGTAAGGTGA11.4084507042253522No Hit
ATTTCAAGCGACCTTGCTCCTCGGTCTTACCAGATATTGACCGACCCAGA11.4084507042253522No Hit
TGTGTTGGTGAGAGCGGGGGCGTGGGCGGTCGGGGTCTACGGTCGCTGCG11.4084507042253522No Hit
GATCAATCTGCCTCGTAGCCGGCGTGCCCATCCCTTCGACGGCTTTGGTC11.4084507042253522No Hit
ATGTACTTCTGTAACATATTGAAAATGACAATTATAAAAATTCAAGTGGA11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG50.070.065
CGACACC50.070.012
CAAGAGT50.070.018
CCAATAA50.070.05
CAGTTGT50.070.062
TTTCCTG50.070.024
TGGTAGT50.070.029
AACGACA50.070.010
TGTCTGG50.070.066
CAACCAA50.070.02
TCTGGAC50.070.068
AAGAGTT50.070.019
TCGCGTC50.070.047
TAACGAC50.070.09
ACCAATA50.070.04
GTCTGTA50.070.051
TGCTGAA50.070.040
GTATCAT50.070.055
ACACCAA50.070.014
ACCAAGA50.070.016
Produced by FastQC (version 0.11.5)