FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_WRKY40_c24_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_WRKY40_c24_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304269
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG6450.015.19260770
GTAGGGC2454.2790743E-614.28459370
GTAGGGG5853.6379788E-1211.96487370
CGGTGGG2701.6750548E-411.66575170
GCGTCGG4904.5794877E-810.71344570
GAGCGTG3000.00523061359.33260270
GGACAGG3700.0030192948.5140056
GTGGCCA3750.00341399978.39908663
CTCGGTG5058.133491E-58.31617970
TCGTGTT29900.08.31038570
CGGAAGC10302.8012437E-107.81492170
GCGTCGA5956.511576E-57.646574562
TGGAGGG18800.07.44622470
TCGGTGG5350.00136611777.19569770
AGTGTAG25850.06.769294368
AGAGTGT34200.06.753855770
ACTCGCG5900.0038259786.52540527
TGTAGGG72050.06.411710770
CGGGCAT7156.216262E-46.3625665
AAGAGTG45750.06.349228470