FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_TGA5_col_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_TGA5_col_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862349
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2800.021.2474570
CGGTGGG1951.830449E-819.7412270
AGAGCGG5150.013.59060170
CGGAAGC7850.012.92838470
TCGGTGG5104.3655746E-1112.35145869
GGAGGGG3407.337039E-712.35145870
TCTCGGG2601.17844495E-412.1139370
GTAGGGG5551.9099389E-1011.34998970
CTCGGTG4702.9698458E-710.42428168
GATCTCG8101.8189894E-129.937077570
AGCGTCC2850.00342514749.82338170
GGTGGGG2850.00342514749.82338169
TCGTGTT29000.09.05063870
GTGGTCG3500.00182580498.998919570
TCTCGGT7251.9308573E-88.68814667
GAGTGTT11850.08.56437370
GGTGGTC3700.0030228818.51249169
CGGTGGT5401.9369767E-58.42491469
AGTGTAG29600.08.39426269
GTGTAGA27950.08.13855869
Produced by FastQC (version 0.11.5)