FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_TGA5_c24_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_TGA5_c24_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446540
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14620.10106875717228697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC1403.2873686E-722.50055544
GCGCCGG952.7814237E-422.10580846
GGCGCCG1601.186565E-619.68798445
GCGTCGG14800.019.3896970
GGTCGCC1953.9504266E-717.94916244
GTCGCCG2551.1277734E-917.84357845
CGGAAGG5200.016.82501270
GTGGCCG1554.0552864E-415.80684345
GTCGGCG2051.2591967E-515.36623245
CGGGGGT2456.754917E-512.85657140
GGTGGCC1950.002300343212.56441344
GCCGGAT2408.1702427E-411.66695448
CGGTGGG3104.802032E-511.28982140
GCGTGGG4501.6302874E-710.88765270
AGCGCGG4207.8868106E-710.83210370
GTAGGGG11800.010.67675370
GAGCGTG9050.010.05410770
AGAGCGG27200.010.035624570
GGTGGTC4251.0650481E-59.88191341
GTGGTCG4451.8369743E-59.43778242
Produced by FastQC (version 0.11.5)