FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_MGT1_col_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_MGT1_col_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4737349
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG9200.020.15976370
GCGTCGG5800.015.68710270
GTAGGGG9250.013.61940670
CGGTGGG4355.7661964E-1012.87146970
CCTGGAC4501.3078534E-811.66427764
CGGAAGC16950.011.14862870
CTGGACG5251.2607416E-79.99784665
GGTCGCC3551.9015573E-49.85755169
GTAGGGC4401.611764E-59.54390170
TCGGTGG10850.09.35331670
TCGTGTT56350.09.06684870
TGGTCGC5502.5412592E-68.90764170
TCGGGGG3150.0078163788.88744368
AGTCCGG9151.8226274E-98.033438
GGACGAT7959.23701E-87.92282167
ATCTCGG20850.07.88840470
TCTCGGG10809.458745E-117.77651270
CAGTCCC4550.00224469727.69235548
GTCCGGA9302.08438E-87.5274849
GAGTGTT22300.07.37548170