FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n02_MGT1_c24_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_MGT1_c24_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAGCTTTTTAGCATAACACAAGATGTTTTTTATAGAAATTAAAAAAC11.8867924528301887No Hit
AAGAAGCAGAAATTATGATTGTAGGTGATAGATTTCCCAATATGAAGAAA11.8867924528301887No Hit
TATATAACCTATCACATTTTGTTATTGATAACATCCCAAGTGAGACCATC11.8867924528301887No Hit
TTTACAATTGATTAGAGTCTTTGGATATAAATTACGAGAATCTATAATCT11.8867924528301887No Hit
ATTCTGAACTAATTTACAAAATCTGTCAGTACATAGTTACTTGGTCGTGC11.8867924528301887No Hit
GTTGGACAACATCTTCGAATATCCGGTCTCAGATTCTGTTTAAGACAACA11.8867924528301887No Hit
TGCGTCGTTTTTGGATGTTTTTGGCGAGCCAGTTGATAAATCATTGCTAC11.8867924528301887No Hit
ACTGAACCAAGAATTCTCTTTCTTTATCATCAATCGAATCACTGTTCGCG11.8867924528301887No Hit
CTGGTTATCAAATGATTGAACAACCGGGAACAATTTACTTACGATACTTA11.8867924528301887No Hit
AAGCTTACCCCAGACAGTGTTAGACCCGAGTGTAGATTTTACGCAATCAA11.8867924528301887No Hit
AAGATATTCACTTTCATCTGCTCGCTTCTTAATTTGTGTATCTCTTTTTC11.8867924528301887No Hit
GTATGTTTCTTCTCTTTGTCACACTATTTTCTGAGGTCTTTAACTTCAAC11.8867924528301887No Hit
GTTTCTCTCCCGCGCTTATTCTACGAGTGATTTACAGTTGTTACCTCGAG11.8867924528301887No Hit
AGATCTATTAAGCACACCGAATACTTAATGATCTTCCCGATACTATAAAA11.8867924528301887No Hit
GAAGAGCGTTCTTCTCGATAGGCCAACGAATCCAGGATTCTTTATGAGCA11.8867924528301887No Hit
GTATCAATGAGGCTCGGCGCGACTTGGAATCCCGCGTAGTTACAAGGGCA11.8867924528301887No Hit
CTTATTTTTAAATTAATAGAAAGACTCGAAGATTGCATTTCCATAAAGTC11.8867924528301887No Hit
GGAGTCAATTTTTTTGTTCAGGGAAACACGAAACCACAAGGCCAATAAAA11.8867924528301887No Hit
CTGCTCTCTTCTAAATTTGTGTATGTCTTTTTCGAAATGGAATCCAAGAT11.8867924528301887No Hit
CAATAGATAAACATCGTAATAAGAATCTATGGTATTACTTTGTGGAATCT11.8867924528301887No Hit
AAGATACATTACTCCTGTAAATTCGGATAATCAAGGAGGTAAACAAAAAA11.8867924528301887No Hit
CTTGGGGACAAAATGTTTTGGATATAATCCGTTCATCAAAATTGAACTCT11.8867924528301887No Hit
CGTGTCTTCCGGTTCTTAAGATTCTCGTCCTTGAGTCGATTTGGTTTGAT11.8867924528301887No Hit
GCAGAAATGTGCTGTTCAAGAATCCATGATACTGCAAGGATCTTCTGTAG11.8867924528301887No Hit
ATTTCGAACTGATCTCCAGTTCCGAAACCAGGAACTTTTCCCCAAGCTAA11.8867924528301887No Hit
AGATTGGGTCGCAGAGCGTAGTGGGGCAGACACGGTTCGTTTGGGTCTGA11.8867924528301887No Hit
CGTCATCTCCTACTGCCGCGCATTTAACCGGAGATTTTCAGCTGATTCGT11.8867924528301887No Hit
GAGTAAACAGAGAGATGACTTACGAATTCGGATCGGATTTGATGATATGA11.8867924528301887No Hit
CCAATCCAATGCACTTGACTAATTATAAGAGTTAGAGATGGATTAAGAGT11.8867924528301887No Hit
ATAAACGACAACCCTATCTCATAAGCCTAAGTAATGTATCCTTGTTACAA11.8867924528301887No Hit
AAGAATCTTGAAAATTTTAACACTTTTTCTAATTGAAACAATTAAGAGAG11.8867924528301887No Hit
AACTAACCAACATTTAATAGTTAAGTTCAATTCATTGTAATTGTGTTCTT11.8867924528301887No Hit
GAATCAGAGCTTCTTTTCATTTGGGTTACTCAATTGTGAAATACTTAAAA11.8867924528301887No Hit
CTAAGTCCATTCCCATTTTGCGCGGAACCGATCTACTAATTCTTTGATTC11.8867924528301887No Hit
ATTTATTAATTTTTAATTCAATTAACTTGGGACAATATCTAAAATTGCTT11.8867924528301887No Hit
TAAGTTTCCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTG11.8867924528301887No Hit
GGGCTGGATAGACCAACCACCGACGCCCCTTCAAAGGGACCATAAAACAA11.8867924528301887No Hit
CCCGGAACTGGAGCCCTTAGATACTGCGATTTGTGACTCCAACAATTGCC11.8867924528301887No Hit
CATGAACTAGATCCGAATCATTCTCAACGAATCCATAAGAAGTGATCCTA11.8867924528301887No Hit
AAATAGATATATAATCAAACTTAATAAACCACAGATAATACAAAAAACAC11.8867924528301887No Hit
ACATAATTCCATACCATAGTATCAAATATAGTTGATATAAGTATTACATG11.8867924528301887No Hit
TAGCCATGCCGTGCAAAATAGACTTGAGAAGTTCACCGGCGTTTTACTAT11.8867924528301887No Hit
ACGAAAAACTTCTCTCAAGAATTCTCTATTCTAATGGCTAAACCTACTCA11.8867924528301887No Hit
ACTGGAAAAAGAGGGAGAGCGTGGAACAAAAAAAATCTTGCACTTATAAG11.8867924528301887No Hit
GCAATCAGATCTTAAAGGCCTAAGTAGTGTTTCCTTGTTAGAAGACACAA11.8867924528301887No Hit
AGTAACTTTTGATGAAGTAAACCTCCAAAAATACATAAACTAATAAAGAT11.8867924528301887No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAA11.8867924528301887No Hit
GACACAAGCTCACTGTACATTCTGGTGCAGCCATGGAAAAAATAAAATGA11.8867924528301887No Hit
CATTTGCATAGGGGATAATGATGATTTGAAACCGATAAGCATGTTTTGAT11.8867924528301887No Hit
TACACCTAATGGTAGGTATTGTAAAAATTTAATAAATAAAAGAATATTTA11.8867924528301887No Hit
TAGACTCCTTTACTTGAACGAACCTAGATCTTGTAGAATCATATTGAATA11.8867924528301887No Hit
GCCTGGTTGTCATGTGTATGATTGAGTATAAGAACTTAAACCGCAACCTG11.8867924528301887No Hit
GTTATATGATGACTCAACTATTGTCTGGTCGAGGCTTTTTGAAGAATTTG11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCAT50.070.060
GAGAATC50.070.036
TTCATTG50.070.062
TCCTTGA50.070.051
AATCTTT50.070.057
AATCTTC50.070.046
ATCTTTC50.070.058
TAAATTA50.070.028
AATCTAT50.070.039
CATTGAA50.070.064
ATCTTCC50.070.047
CTATAAT50.070.042
AAATTAC50.070.029
GATATAA50.070.024
ATTAGAG50.070.011
ATCTATA50.070.040
TATAATC50.070.043
TATAAAT50.070.026
TGATTAG50.070.09
TTTCATT50.070.061
Produced by FastQC (version 0.11.5)