FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_MGT1_c24_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_MGT1_c24_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333455
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGACG4700.017.8701165
CGGTGGG2954.3837645E-1016.60886470
GGTCGCC4100.016.21917244
GCCGTAT4300.015.46479248
AGAGCGG12150.014.97824770
GCGTCGG6300.014.44330970
GTAGGGG10150.013.79201970
TCCCTCG10000.012.59828231
CCTGGTG8350.012.57458850
CCTGGAC5307.2759576E-1212.5454664
CCCTCGA8950.012.51269432
GGACGAT7350.012.3795567
CGGAAGC19350.011.75618170
CCTCGAC10200.011.66561433
CTGGTGG10200.011.322638551
TGGTCGC5401.4606485E-911.01752643
CGCCGTA2550.001358411610.98016347
GTCGCCG4551.9001163E-710.76900545
CCCCGAT2750.00254239910.1837213
GGTGGCC2750.002546785610.18160444