FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_LHY1_col_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_LHY1_col_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4902642
Sequences flagged as poor quality0
Sequence length76
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG4950.017.6748370
GTAGGGG7250.013.51575970
CGGAAGC12600.013.3318770
AGAGCGG8150.011.5938270
GTCGGGG3055.1805424E-410.326735570
AGAGTGT29800.07.39851770
TCGTGTT31250.06.383299470
GTCGTGG41800.06.195492770
ATCGTAC6800.00245611166.176171338
TCGTGGT13651.5024852E-86.153170670
GTAGGGA37200.06.1149268
AAGAGTG39800.05.979243370
GTGTAGG56300.05.90521470
GGGAAAG38100.05.786353666
TGGAGGG21351.8189894E-125.737075370
GAGTGTA24250.05.483934469
TAGGGAA41950.05.42252869
AGTGTAG24801.8189894E-125.362314770
TGTAGGG51650.05.35275270
CGTGTAG61600.05.28351170
Produced by FastQC (version 0.11.5)