FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28074332
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283920.10113152469665174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG57750.022.00928142
CATTCAG185750.021.36330433
CACATTC220750.018.06990231
TCGGGGG55100.017.7302143
AATCGGG73400.016.07545941
ACTCCGG79250.016.07160432
GTCACAT263850.015.51309629
CACTCCG85550.015.46049731
TCCGGAG88200.015.23599534
ACATTCA276600.014.73773832
CTCCGGA91250.014.30408733
GCCGTCT34050.013.7680646
TCACATT297150.013.67107930
AGTCACA370050.013.22595328
CCAGTCC84050.013.21190970
TTCAGAA305750.012.79725435
AATGGGG63050.012.77239241
CAGAAAT264300.012.26294237
ATTCAGA338550.012.063024534
TATGCCG46000.011.8645673
Produced by FastQC (version 0.11.5)