FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n01_coldna_daplib_dapv1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_coldna_daplib_dapv1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGAGATAGGCGGACTGTGTAGTCAATGCACGAACTGGCAGCTAGGCG12.272727272727273No Hit
TCAGCCCGTAACCGCGCAAAGAGAATTTTACATAATTGATAGCGGACGAG12.272727272727273No Hit
GCTTAAGTCCAGTCGACAGAGGAAAAGTTGGCAACCGTGATTAAAAGCTA12.272727272727273No Hit
ATGCCTCCAGAAGCTGTTGATTTGGTCTCAAGACTTCTTCAATACTCTCC12.272727272727273No Hit
CTGAATAAACAGTGTCACCATATGTTGTTGAGCCTTTCTCTGCACACAAC12.272727272727273No Hit
AATAGATACGATTACAATGCAAGTAGTAAAAAATGAGATCATACAATCAC12.272727272727273No Hit
ACTTACAGCGGCTGGACACACAAAGGCTTAGAGAAGAAAGAACAGAGGTA12.272727272727273No Hit
ATGTCACTATGAATCAATATCAGGAGAGATGCTCCCAATCCAGCCGTTTA12.272727272727273No Hit
AATTTTATCATTCTTAAAAGACAAAAAGAAATACAGACATATAGAAATTG12.272727272727273No Hit
ACATTATGTGATGATGAAGTTTGTTGCAACGGCTAAACAGAGAATATACT12.272727272727273No Hit
CCTCCGGATCAATCGCCGTTTAACGAGCCAAAACATACAAAACTGACAGT12.272727272727273No Hit
CCAAGTTTAACTCTGACGTCGCTCCGTTCTTTTAGGGTGTGAAGCTGAGT12.272727272727273No Hit
TTGGTTAGTGTTTTGGAGTCGATTATGACGTGATGTCATGTGTATGGTAG12.272727272727273No Hit
CTGCGCGTCCTGGTTTTGCAGAGACAAACCCACAGTCTACACAGTCTACT12.272727272727273No Hit
AATAGTGTGGATACTACAAAATGTGTAAAATATCTTTGACGTTATTCTAG12.272727272727273No Hit
TTTAATCGAGAGATTCATATTAAATGTTTATAGTGAATGAAAGAGATTAA12.272727272727273No Hit
ATTTGGGGTTTCGATTCTAATCTATAGATAAGGAATAAAACCTACTCCAC12.272727272727273No Hit
CCTAGTTAGAGGCCAATAAAGAACACCAGATACTTTAAGATTCTCGCATA12.272727272727273No Hit
TAATTCACCAATCATGTCGGTGTCGAATTTTAGGTTTTGAATACAAATTG12.272727272727273No Hit
CAGACATTAAATATCACGTTAAGCGCCATATCTAGACTATTTTTCATCTA12.272727272727273No Hit
AGCAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGAATATGACTTGAT12.272727272727273No Hit
CTGATCCGCCCTTTAGCTACACGGTCCTCCCTTTTATCCGAATCACTGCG12.272727272727273No Hit
ACAAGTCACTAACAAAAACCTGTGCACTTTTTGAAGGTATCCATGATGGA12.272727272727273No Hit
AACATCTTTTCATGATAACATTTCTCCCTAAAAGAGAAACAAGAGATTAG12.272727272727273No Hit
CAAACAAAACCAAACAAAAATAAAGCCAAGCCGACTAAGATGAAAGATAT12.272727272727273No Hit
CCCAGAAGAACACGTCCTCCCAATACAAGTCAGCGCGGATAAAGACGACC12.272727272727273No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAACAACTCGCCTAG12.272727272727273TruSeq Adapter, Index 1 (97% over 37bp)
TCACCCACCTTCTTCTTGCTTCTCAAAGCATTCATCGTGTAGCGAAAGTC12.272727272727273No Hit
AAACTGGGCGTGCATTGCACGCGAGCGTATACAATCGCTCACGTTGATCC12.272727272727273No Hit
TCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAGACACAAAGCCAAACA12.272727272727273No Hit
TTTAAGAACTTATACTCAATCATACACATCACATCAAGTCATATTCGACT12.272727272727273No Hit
CATACACATCACATCAAGTCATATTCGACTCCAAAACACTAACCAACCTT12.272727272727273No Hit
AACACTCATATGGACTTTGGCTAAACCAAGAAAGCAATGATAAGCAAGAT12.272727272727273No Hit
AAGGACGTAGAATCCTCATCTTTGTTGCCATGAGCCACCCAAACTCAAAG12.272727272727273No Hit
TTATATACAACCATATCGGATGGTTACAAATGGGCGACTGATGGAGGCAA12.272727272727273No Hit
TAACTTTGGTTATGTTGAATGAATGCTTTAGCGGAAGCACCCCAAGCTAG12.272727272727273No Hit
ATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATACACATGACATCAAG12.272727272727273No Hit
TGATATATAACTCCTCGAAAAATAATTTTACACCACTCTATGAAATTCAT12.272727272727273No Hit
TAACTCAGTGCCGTCGTTGTTAGTTGTGTGTTGCGTAGTGAATGAATTTG12.272727272727273No Hit
TTTTGAGGGTTGGTTGAGGGTGTAGATCTGTAGATCCTTCATTATCTTGT12.272727272727273No Hit
TAGGCTTACAAGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATACA12.272727272727273No Hit
CTTTCATGGTGTAGCCAAAGTCCATATGAGTCTTTGGCTTTATGTCTTCT12.272727272727273No Hit
GGTAGAAAACTATTTAGAGTGGCCTGAATAAATAGAACTAGATGATTTCC12.272727272727273No Hit
GTGCTGCTTAGAGTCTATGATAGAAATGAGATACCATCAGATTCGAACCC12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)