FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n01_abdna_ablib_dapv1-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_abdna_ablib_dapv1-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401530
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG200.00695250852.49970620
CGCGGAA1056.766113E-726.6722281
ATCGGGG1755.2550604E-921.99987670
CGCGGTT1055.4675876E-419.99988770
TACAGGC900.005276513319.445032
CCAGTCC4350.019.31023670
TGCCGTC1351.412783E-418.14804548
GTGGGCG1000.00969171217.49990355
GCGGGGT1250.001762920816.79990650
ATCGCGG3601.8189894E-1216.52768570
TGCCGGG1807.1529816E-515.55546948
TCGCGTG1400.00375460914.99991670
TATGCCG3051.2119926E-814.9179546
CTCCAGG2951.3023237E-714.23720870
CGGGGTG1500.00593631413.99992151
CCCCCTC1550.007365314813.5512121
GTATGCC4152.7102942E-1013.493945
AAGAGCC11350.013.25983770
ATGCCGG1850.001548582913.24316947
CACACGG2456.752169E-512.85707170