FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n01_WRKY40_col_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_WRKY40_col_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCTCCCACTGAATTGAATTGGGTCCATGAATCTAAGAAATAGTGAGC14.0No Hit
AGGTGCCAGGACAAATCGGGCTTCAAATATATCGGGTCTCACGGTGCATT14.0No Hit
ACAATATGAGAAAGATTAGTACTTAGGAGGACTTATTCCCCATAGATTCC14.0No Hit
CTTATACTCAATGATACACATGACATCAAGTCATCTCCGACTTTAAAACA14.0No Hit
GTTTTGATGATTAATAAGAAGGTATTCACATCGAGTATCTCTTGACAATT14.0No Hit
AATATGCAGTTATTTATTTAACCTTTTTCTTTGGTTTTGTTTTGTATATG14.0No Hit
CATAGAATCCTAATGTCAGAGATTATAGAAGAAAGAAGCTGTGCAAGTAG14.0No Hit
CCTATAATGCGGCAACCTTATGGGCACCTCATGAAAATATTGTCACGCGA14.0No Hit
TTAGTAACAGTGAGCAAACGAGCATTTAACGAAATCTACCCTTATGGATT14.0No Hit
TTGTAAGCTCGAATTGCGGTTGAAGGTGTTATACTCAATCATACACATGA14.0No Hit
ACCGGGTAATTAACGTCTTGAGAAAAAATAAAAGGTTATATTCAAAGAGA14.0No Hit
CCTTGTTAGAGGACACAAAGCCAAACCATAAAAAGTAATAACGATAAACC14.0No Hit
TTTATTACTTATATTAAAAAGAAAAAGTTAAATAAATAATGTTATATACG14.0No Hit
AGCCTTCCTCGACCGGGTCCGAGGATTTGTCGACCAGGCGTTGAAGTCGG14.0No Hit
CATCGCACTCAAGAGACTATACTACCCGCGACCAAATGACTTGACATTGA14.0No Hit
GGCTAAGCATCTCCTTTGGGCATAGTATTCCTGATACCACAAACAGGGCA14.0No Hit
CGGGGCGGAGAGATCGGCGCGCGGGCCGGCGGCCCCGTCGACGCGGCCGC14.0No Hit
GTTCTTTAAGCTATTTTTTTTCAATAAACAAACGATTCTGACTGTGTTTA14.0No Hit
GGGGTGTCAAGGGGAAGGCCAGCCCCTCCCTAGTCGGGTGAGTGGGGAGG14.0No Hit
CTTACGAATTCTTTCTTTCTTGTTTAAATAAGGGTTTGAATTAGATAAAA14.0No Hit
TGTCTTGATGTCATGTGTATCATGGAGTATAAGAACTTAAATGGCATCCA14.0No Hit
TTCACCTTGACTTTCATCTCTGAAAAATGAAAATTTTACATTATTCTTAA14.0No Hit
TTGATGTCATGTGTATGATTGAGTATAAGACCTTAAACCGCAACCGGATC14.0No Hit
GGGAGATAATGCTGTGAAAAATCACGCGGGTAAGAAAACGCTCGTGAGCT14.0No Hit
AGAAATACGACTTTGCACTATAGTTAACTCAGCACCAATCAAGAATCCCC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)