FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n01_WRKY40_c24_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_WRKY40_c24_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAATGCGGTTCCATGGGAGACCAAGAAGTCCCGTTCTTTCTACTCAAT11.4084507042253522No Hit
GACCCTCTTTGACTAGAAAGGCTAAAGCCAAGAAAAAAAGAAGCTCGAAT11.4084507042253522No Hit
GTCCGAGGATTTGTCGACACGGAGTGGAAATCGTCGAGAAAAAACTAACG11.4084507042253522No Hit
GCCCCAATACAAGTTAACAAACTCATGTAATAATTCTCATTATTGTTCCG11.4084507042253522No Hit
GCGTCAATATCTGGACTCTTATATCTGTAAATCCAAATATTTTGGTTTGT11.4084507042253522No Hit
ATGGACCAAATGTAGGAACACCGGACGAATAACCCAAATAACCTATCCTC11.4084507042253522No Hit
AGCTTCCTTTGTGGAAGACACAATTTTTTCTGAAGAATTCGCCATGATAT11.4084507042253522No Hit
GCGGCCTTCTCAGGATCTTCAAGGCAAAAGAACGAGACTTTCTTCACCAG11.4084507042253522No Hit
CCGCACTAGAAAGGCTAAAATTACTATGCTACTTATAATAGAAACTGCCA11.4084507042253522No Hit
TATCATGTGATTTATTAAGAGTTAAGGGATTGATTTATTTAAAAAAAACA11.4084507042253522No Hit
GGGATTTGTATATGCACATCATCTCCAACATTGCCTCCACATTCGTGAAA11.4084507042253522No Hit
TCCTCAGGTGACAGTATTTGAGGTTTGTACCTCCCCCCAAGCTTGTAAAA11.4084507042253522No Hit
TGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAG11.4084507042253522No Hit
CCTTGTTGGCGCCCCCGCCCTGCCAGCAGAGTCGAGTCCCTCGCCCACAC11.4084507042253522No Hit
GAAACTAGCCAGCAAAAACGGTCATTAAGATTTCAATAGTCCTTTAACCC11.4084507042253522No Hit
GCGCCTGGTGTTGAGATTCTTGTTACTCTAATTGATTTCGCTGCTGGCAG11.4084507042253522No Hit
GAATCCTCGACAAGCTCCTCACCATCATACACATTTTCAAAAGAAACCAC11.4084507042253522No Hit
GGCGAGACCAAGGGAGCAGAGAGAACAAAGAAAGAATAAGCTCAAGGTAG11.4084507042253522No Hit
GCGACAGCTGTTGAGTCAAGAGTGGAAATTGATGGGAAGATAGTGACGAG11.4084507042253522No Hit
CGCACTCTAAATATCCTTGAAATTTATCCATGTGGATTGAAATTCGATTT11.4084507042253522No Hit
GTCGGTAGCTCGGCGGTTGCGTGACTAGGACGGACGTTCCCGCCCGAATG11.4084507042253522No Hit
TGGTCGGTTTGGTAGCCGCAGCAGTAAACATGAGTGAGGATAAAGGAGAT11.4084507042253522No Hit
TTCGTTCGATGACGTGTTCAACAAGAAAACGTAAGGTTCGGTGAGTGAGA11.4084507042253522No Hit
TACGCCTCCTCTTCCTCTCTTACTCAACCATTCATTTGCCGTCCACTTCA11.4084507042253522No Hit
AATTGTGTCTGAATAAAAGAACAGCAATTATATGCTGAATACTAAAATAA11.4084507042253522No Hit
ACACAAAGAACAAGACTCATATGGAATTTGGCTACACCATCAAAGCTTTC11.4084507042253522No Hit
CGGTTAATCTTGTTATACTCAATCCTCCAGAATAACACAAGAAAAATTTC11.4084507042253522No Hit
TTAGCGCCCCTTTTAAAAATTTGAAATTTATATTAAGTTTCGTGGGTGTT11.4084507042253522No Hit
ACCAACTAGACAACGGTCAAGAAGAGCGGGCTTATTTAAAGAGTCCAATC11.4084507042253522No Hit
ATCGGTCAAGAAAGATAAGAAAATTTCAGGGTAGCAAACATTCTCTAGAA11.4084507042253522No Hit
CAACACTTCCTTAGAAGCAGTCAATAGATTTGTTACTGTTTATATCCTCA11.4084507042253522No Hit
CGATGACCGAGACTTTCGAACGGGCAGATCAAACACTCAAACTCAAAATG11.4084507042253522No Hit
CCTCCCCAAGGCACCCATCCAATCTGAACTTATAGACATCAAGAAGCAGC11.4084507042253522No Hit
TACGCTAAAATGATAATAAGTCTATGCGATAACAACGTAACAAGAACAAT11.4084507042253522No Hit
GAGGGGGTCATGTAAATTCAATGAGACTATGGATCGTTCGCTAGCGGATG11.4084507042253522No Hit
GCTTGGGTTTCCCTTCAATTTTGAACACGAAATCTTTCCCCCCAAATGAA11.4084507042253522No Hit
AGAGGTAAGTTGCAATTATGTGAAGGTGTATCAACGAGAAGACCTCTAAG11.4084507042253522No Hit
TTTTGCACACTATTCAACAGCATCCACGGCTGTGTCGCAAAAAGCAGCCA11.4084507042253522No Hit
GGTCGATGAAGTCTCGGCCCTGGTCGACGAGTTGGCCCCCTGGTCGCGCA11.4084507042253522No Hit
ATGACTTTGCGTTCGACTTTGGTCTTAGGGATAGTGATAAAGAGGACTCC11.4084507042253522No Hit
AACCCGCAACCGCATCTTAAAGGCGTAAGAATTGTATCCTTGTTAAAAGA11.4084507042253522No Hit
AGGGTAGAGACTAAGAGAGAACCAGGACTTAGTCAAGCGTAAAAATCTTT11.4084507042253522No Hit
TAACAAATAAGTGCAATTTTTAGTTATTATAGATTGCCTAAAAAAAAACC11.4084507042253522No Hit
CGGATTTCTCGACCAGGAGATGATAACGTCGCACCGGAAGCGGAAGGCAA11.4084507042253522No Hit
TTTAACGCATACAACTCAAGAAAGCAACAGGAAGCAAAAGAAAGAACACA11.4084507042253522No Hit
TTATAGTGATTTGACCAAAGCCGTCGAAGGGATGGTCACGCCGGCTACGA11.4084507042253522No Hit
AAACCCTCTAGAATAGTGTAATCATCAAAAGTCTTGATTATGTTAATTGA11.4084507042253522No Hit
CATCTGTAGCGCTCCTTTCGGTCATAGTTGCTTCATAATCCCTAACAAAT11.4084507042253522No Hit
CCCTACCATTTTTTATTTTCTTGTAGTATTGTACAATTGGGAAGACATCT11.4084507042253522No Hit
TGTCAACCTTTTCGGGGAGTAGAGCTGGGGATAGAGGCCCTTGAACCCTC11.4084507042253522No Hit
AAGTGGGTATTTATAAAAACGATCTTTACATACAGAATCGAAAAGAGATA11.4084507042253522No Hit
CGGACCGAGCGTTTGGTTTGTGTGACACCTTTATTTCCATATAGAGACTA11.4084507042253522No Hit
TTATAAGAAATTAAACCTCAAACTTATCTTTAAAGCCTATGTAATGTTAA11.4084507042253522No Hit
ATATGCTGACCGGATAGCTGCGACTCGAGCGACGGCCTTCCCCTGCGACT11.4084507042253522No Hit
ACCAAGTGGCAGAAATTTTCATCCAGTTTGGCGGAATGTGATCTTTGGTT11.4084507042253522No Hit
GGTGCAAGGTAAGCATGAAATTATTTATTTAAAACTATGATTTAGATATT11.4084507042253522No Hit
GCCATGCGGACTTGTCAAAGACTGAGTATACCTTCAGACCCAGCACCCCG11.4084507042253522No Hit
CTTCGTTACTTAGTTTCAGGCAGCTGCGTGGGAAAACACAAGAATCCCAC11.4084507042253522No Hit
TGGCGGTGGCTGTGTGTCGTCTAAGACGGTGGCGCTAGGGAGGCATGAGA11.4084507042253522No Hit
TCGGCGTCCGTGTTGCGGGTTAATTTCTTATTGTCTAACATACAGATGAG11.4084507042253522No Hit
GCATGATAATGAAGATACTAGCACTCCAGACACTGTTCTTGATGGGCAAT11.4084507042253522No Hit
TGAAGAATTACTTGCTGCCGCAGCGTCACAACTCGTGATAGTTTTGGTCT11.4084507042253522No Hit
CCCTTGGGGATGCGGTAATTCATTTAAGAAAGTATACCATCGAACATATT11.4084507042253522No Hit
TAACTAATAATTTCGAGTCAAGAAAATGTTAACAAGAATTTCATACGTTT11.4084507042253522No Hit
GATGCCACGACGACTTATTAGAAATTTTCTTCTATTCTTCACCTTCAACT11.4084507042253522No Hit
GCTAGACATGCTAAGTACTCCCCAGTGATCATGGTTTGCTTCTGTACATA11.4084507042253522No Hit
GTGGAGCTGGTTGCATGCCTAAAGTTTATTAATTACGTGGCACTACCTGA11.4084507042253522No Hit
TGGAGGTTCTCAAACGTTACAAGAATTATAAAAAGTCAAATTGATTTATT11.4084507042253522No Hit
TGAGTTTATTCCTCGACCATCGACCAACACCATATGCGCTGCCGTTCGAA11.4084507042253522No Hit
CCTGCAGTCAATCAGGACCCGCCATTTAAGCAGATCTACAGGAGATGGAG11.4084507042253522No Hit
ACCGACCGTTCCGACGGCACCCCGGACCGATTTTGATTAACAGACCGAAA11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATA50.070.030
TTCATTT50.070.019
GGATGCG50.070.08
GGATGCA50.070.058
GAGCGAC50.070.069
CCGTGGA50.070.054
TATACCA50.070.033
AATTCAT50.070.017
ACCATCG50.070.036
GCAGGCA50.070.062
GATGCGG50.070.09
TCGAACA50.070.040
GATGCAG50.070.059
GCGGTAA50.070.012
ATGCGGT50.070.010
CCTTGGG50.070.02
TAAGAAA50.070.025
CATTTAA50.070.021
GTATACC50.070.032
CTTGGGG50.070.03
Produced by FastQC (version 0.11.5)