FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n01_WRKY40_c24_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_WRKY40_c24_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304269
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC8450.016.56785670
CACACGG4304.783942E-1013.02310770
CGGAAGC14850.012.4914470
CACGCGC2050.003348984511.95108257
CACGTCG4802.7121132E-911.666532570
CGATCAG20300.010.51712170
CACGATC28500.010.19286669
CGTCACG3451.4228227E-410.14481239
ACGATCA27650.09.99988670
CCTACCC2950.0045413159.4940021
CCCCCCC2950.00454755559.4924222
GCAGGCA2950.00454755559.4924225
GATCAGC8101.6552804E-109.0739770
GTCACGA38450.08.82954869
GTCACGC5805.191643E-68.44817970
CGTCTGC7151.424105E-78.32158370
TCACGAT36050.08.25233368
AAGAGCC15400.08.18172470
GCACACC5603.0550906E-58.124907570
AGCCCAG4000.0060927437.8757442
Produced by FastQC (version 0.11.5)