FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n01_WOX11_col_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_WOX11_col_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATGAAGTGTATGATTGGCCGATAATCAAGGTGTAGCATTTTTTTACG13.571428571428571No Hit
AAGTTTAATTGTATTCTACTTTATTACCCTTAATTTCTGTTTGTTGTCAT13.571428571428571No Hit
TAGTATATGTGAATTACGGGCGCAGTAATCATTGTTCACAGTTAGGGCAG13.571428571428571No Hit
TGAATTGCTGCGATTGAAGATAGACTAACAACTCCGAAAGCAATTGCCAA13.571428571428571No Hit
ATTAGTTGAATTAACTCTTTCAAAATTAATTGCTAATTTCCTAAAATCTC13.571428571428571No Hit
TTATATTTGTATACTGATAAGAAAAGGTTATTGAAAGCTTAACGTCTTGT13.571428571428571No Hit
TTTATCAACTACTTATAAATCTAATCATATCAACCGTCTCCAATTTATTT13.571428571428571No Hit
TCTTTTAATCACAATAATTAGTTACTGCATAATTCCAATAGTGCAAGATC13.571428571428571No Hit
GTAAGTTGGTCTAAGAGGAGAGAAAGGAGAATAGAATTGTGCTTCATTTT13.571428571428571No Hit
TTTTCAATTCTGATGTTCTTTGTGTCGTATTTTATAAACAAAAAAGTTAA13.571428571428571No Hit
ATTACACGTTTAATACTTGCTATACGATTAGCGTATTTACAGCATGATAA13.571428571428571No Hit
CCTAAGCAAGCAATTGTATGTGACTATATCAGGCGGGTAACCTTTAGTAC13.571428571428571No Hit
GTTTAGCGTTTGAATGGTGTCTCTTGGATTGGCATCAGACTTATTTATAA13.571428571428571No Hit
AATTGTAGTTAATATTTTGGATTATATTGATTATTAATCGAGGTTAATAT13.571428571428571No Hit
GAAAGTTAATTTTCGGGTCAATGGTGAAACCAAAGAATCGTTCTATTTAT13.571428571428571No Hit
TAATTCGATATCGTTTTTATCTAAATAATCAAGTTATATTTTTAATTTAT13.571428571428571No Hit
ATGGTGTGAACTACAACATTGTAAGGGTAGTTTAAAACTAACTAAATAAA13.571428571428571No Hit
TAACGGACTTAGGAAGAGTAATGAGGATTGTATTCATTCGGTAAGAATGA13.571428571428571No Hit
ACATTGATAAAAGAAAATGAGCCAAACATGAGATCGGAAGAGCACACGTC13.571428571428571No Hit
AATTTAAATTCGAATTACTAGCTAGTGTAGTTAATAAAACAATGAAAACA13.571428571428571No Hit
TATTTTATAAACAATATTTATTATACACTTATGAAAATATTAGAAAACAA13.571428571428571No Hit
GTTACACTGTCAAACTCTCTAATTTCAAGTTTATCATATAGAACACAAAA13.571428571428571No Hit
AATCGAAATATTAAAATTATGAACTGAATTTCCATTAAGATTCGTAGTTT13.571428571428571No Hit
GACCTCGTTAGCAGCATTCTGCATCTCACCGGATATGAGACATAATCCGC13.571428571428571No Hit
AAGTAAGTAATATCAGATTTTAATTAACTTGAATATTTGGAGTGGAGCTG13.571428571428571No Hit
AGTCCTCATTCTCTCCCGCGAGTAGAACGGCGCGTGCAAATGCCAGTAAA13.571428571428571No Hit
TTTACACGTCGGATATTCTTGGTAGTTTGTGATCTTCCTTCAATTATTCA13.571428571428571No Hit
TGTCAAATGGATTTATGTTTATGAAGAATTAAAATTTGTAGAGAATAAGT13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)