FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n01_TGA5_col_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_TGA5_col_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862349
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC500.007212096427.99952523
GAGCACC7200.020.90242670
ACAGTGC4750.018.4207470
CACACGG2401.0308213E-817.49970470
CCAGTCC3552.1827873E-1115.77438270
CGGAAGC10300.012.57260370
AAGAGCC10850.011.61270770
CACACAG37550.010.99848270
ATGGGGG2600.001596503210.76904970
CACAGTG26100.010.72778870
CTGAACG2300.00789278410.65199470
CAGTGAG7351.8189894E-1210.47601470
AGTCACC4301.2239168E-59.76727770
TTCCCCC2950.0045501589.49136545
CAGTGAT16300.09.01825369
TCGGAAC3955.534189E-48.8606170
ACACAGT31650.08.84661269
CCGTCTG4006.2734255E-48.74985269
GTCACAC49450.08.70561668
TCACACA50000.08.60985569