FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n01_TGA5_c24_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_TGA5_c24_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446540
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2800.023.74976545
CGCGTAT1102.924358E-522.27250743
TATGCCG2351.6370905E-1120.85085947
CGCGGAA850.003787652820.591591
GTATGCC3450.020.28965446
TGCCGTC1055.467861E-419.99980249
ATGCCGT2053.2438038E-818.78030248
TCCGTAC1200.001340452217.5016425
GTGCGTG1000.00969209717.49982858
CACACGG2259.4052666E-817.11094370
TAATGCG1250.001761784716.8015775
CACGTCG1906.124539E-616.57878370
TATGCCC1807.153499E-515.55540247
TCGCGTA1400.003754792714.99985242
TGTGCGT1450.004740998614.48261557
GCGTATG1708.177516E-414.41162344
CATCGCG2253.0344343E-513.99986340
ATCGCGT2253.0344343E-513.99986341
TCGTATG4902.1827873E-1112.85701644
GGCTACC3601.0670374E-712.63876370