FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n01_MGT1_c24_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_MGT1_c24_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACTCAACGAATACGATTTATTTATAAATGATCGTGTATGTATATAT11.8867924528301887No Hit
ACCTGCCATCAAGTCATATTCGACTCCAACACACTAACCACCCTTCTTCT11.8867924528301887No Hit
GTGTACGTGACTACTTCATATGGCTGGTCTTCTTGCTGTTGTTCTTGATG11.8867924528301887No Hit
GCGCTTTTAAAAGTCATTATAGGTCCGTTCGCTATTTTGAAACAGACATA11.8867924528301887No Hit
TGTCAAGAAACGTAGAACAATGAAGAATCTTGGGAGAGCAAGGAAAGAAA11.8867924528301887No Hit
GGCTAGTTTTTAGTCTGATCGGTTAAACAGAACAGAATCCAAATTTTGGC11.8867924528301887No Hit
CTAGAGCAACCTGGACCACCATTAAGCCAAAGCACAACTGGAACTACCGA11.8867924528301887No Hit
GCGTGGAGGATCTAAACTCTGCCGTGATTCCATCACCGCAATCAAGAAAC11.8867924528301887No Hit
CACTCCTACAGCCTAAACATTAGGGATTTCTCAACAGTTTAAGTAGGCGA11.8867924528301887No Hit
TGCTGATCGAATCTTTATATTTTTCTCTGTTTTTTTATTCTTATTTTAAA11.8867924528301887No Hit
GTCAGAGACGATCTTCAAGGGTCTATCGAAGCATTAGCTTGGGGAAAAGT11.8867924528301887No Hit
ATCACTAAAATACTCCTAGAGGGGGATAGGGCTAAGCGGAGCGAAAAGGG11.8867924528301887No Hit
GAGAAGACATTTATTCATAATATAGATAGGGTTAGGTCACTTAATATATT11.8867924528301887No Hit
CTCCCTACAAAAAAGGAAACCATATTAACCATTGCGTTTTGAGTTTGTGT11.8867924528301887No Hit
CTTGTATTAAGATATAGTTAATGATTAATTCTATTAATCATGAGATTCAA11.8867924528301887No Hit
CATGGTTCCTTCTCATCCTTGGCAACACGAGCAGCGGTTCCGGCGGAGAA11.8867924528301887No Hit
ATTAGATAAAATGAAAATCCAAGAAGTTGGTTTGTAAAACTATAAATTGA11.8867924528301887No Hit
TATAAAAGTAATAAAAAACAAAAATTAAAAAGATAAAAGAAAAAAAGGAT11.8867924528301887No Hit
TCATAGCTAGATTAGGTAAACGCGGCAACGTATCAGGCTATAACATAGAG11.8867924528301887No Hit
CTTATAATTAGTCAAATGCATTAGATTGTAACACATTTTGACCATAGAAA11.8867924528301887No Hit
GTTTGGGGGATTACGAGATTCTGTCCAGAACCTCCTGAAGTTCAAAGAAA11.8867924528301887No Hit
TGACGGAAGGGCACCACCAGGAGTGGCCCGAGCGGGGTTAATTGACTCAA11.8867924528301887No Hit
ATATACTTATTTAAATTTTAAACTCTTATCAGAACTTTAAACTGAACTAG11.8867924528301887No Hit
ATCAAAGTAGTATAATCAAGTTACAAATTCAAAGCAGGGTGCAGTCTTAT11.8867924528301887No Hit
CGATGGAGGATCGAACCCTGCTCTGCAGCGCCATAAGCAGAGTGTCTGTA11.8867924528301887No Hit
CCTTGATTTTCTTGGATATGTTGAATGTATACAGGTAAATCCATGATGCA11.8867924528301887No Hit
AGAAGGAGAAAACTCACTGGAAGAAGAGGATAGTATAAGTTTATTGCTAA11.8867924528301887No Hit
TCCAAAACACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGT11.8867924528301887No Hit
TTTCACTAATGTTAATTATCAACAATCATAGGTATTGATACAATCATTAT11.8867924528301887No Hit
TAATTTACTTTTTCATCCTAGTTTAAATAAGTGAGAAAACATCAATTGAA11.8867924528301887No Hit
ACTACTCCAACTAGTAAACAAGAGAGAGTGTAGACCATGCAGTTGTTAGA11.8867924528301887No Hit
ATTTGCTCTTAAAGAATCCTGGATTCGTTGGCCTATCTAGAAGAACGCTC11.8867924528301887No Hit
AACGGCCGATTTAGAAGAAAATAAATCGAAGTTTTACATAGTAATAAGGG11.8867924528301887No Hit
AAAATAGAATCCTGGGTCGCGCACAGGGATTCGAGTGATGATAAAGCAAG11.8867924528301887No Hit
TCGTCGTTAGTTGGGGGAAGAATCCTCAGCAAGCGGATTTATTTAGGAAA11.8867924528301887No Hit
ATAGAATATCCCCTTTAAGATCAAACAATTCCAGCGAATTGAGTATGATT11.8867924528301887No Hit
TTAATCCCTTTAATAGTTAAAGGGTCTCTCGGTTTGATTAATCTTCCGAT11.8867924528301887No Hit
ACGATGGATAGACTCTTAACGGTTTTGCTCGAAACAACGTTCGGCAACCC11.8867924528301887No Hit
TGAAAAAAATAGGATCACTTCTTATGGATTCGTTGAGAATGATTCGGATC11.8867924528301887No Hit
CCACCGCGGTGCGGACGTCCGCGAGCCGTTGACCCTTTCCATGCTCCCAT11.8867924528301887No Hit
TGCCAGCCTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCG11.8867924528301887No Hit
CCTTCAATGAGCTCTGTTTAAGGCGAAGGAAAGGCATGGTTAGCACTGCA11.8867924528301887No Hit
CAATTTTAATTCAAAAATTAATTATAGTCTTTTCATTTATTAATCTTCAT11.8867924528301887No Hit
CGGACAGATTTGAACTGAACTTTTGGACTTAATATGCAAACTTGCAGAGG11.8867924528301887No Hit
GTTGAAGTTAAAGACCTCAGAAAAAACGGAGACAAAGAGAAGAAACATAC11.8867924528301887No Hit
AAAGTACACGACTAGGTTTTAGAAAGGCAAGCGGGGAAAAAGAATGATAA11.8867924528301887No Hit
CCCGGCCCACTTAGTTAAACCCAGTGTATACAGCCCAAATAAATAGAACC11.8867924528301887No Hit
ACGCTTTCATGGTGTAGCCAAAGTCCATATGAGTCTTTGGCTTTGTGTCT11.8867924528301887No Hit
GTAAATATTCGCCGGCGAATACTTTTTTTTTAATAAAAGTAATAAAAAAC11.8867924528301887No Hit
ATATGAGTTTCACACTGTTCTTGTGATTACTATCTCTTGTCTCAAGGCAG11.8867924528301887No Hit
GATAAGAAAATAGAGGACACACACCAGATCCGTTAAAGGTTAAACCGTCA11.8867924528301887No Hit
CGGAAAAAACCCAGGGAAGTGTAATTGAATCACCTCGAGCTTACGAACGG11.8867924528301887No Hit
TGAACTTCTAGTAGTTCTTCTTCTAGTGTTGTCTCAGACTTGTGTTGGAG11.8867924528301887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTC50.070.022
AATCTTC50.070.040
GTTTGAT50.070.032
AATCCCT50.070.03
ATCTTCC50.070.041
AAATTTT50.070.054
CTTTAAT50.070.08
AATTTTA50.070.055
TTTCTTA50.070.062
TCGGTTT50.070.029
TTCCGAT50.070.044
TAAAAGG50.070.067
TTATTTC50.070.059
GGTCTCT50.070.023
CTCTCGG50.070.026
TGATTAA50.070.035
AGGGTCT50.070.021
GGTTTGA50.070.031
AAGGGTC50.070.020
TTTTATT50.070.057
Produced by FastQC (version 0.11.5)