FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n01_MGT1_c24_b-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_MGT1_c24_b-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333455
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG4650.018.0642670
GGCGACC1300.002292390716.1536189
GAGCACC12550.014.22290670
CCAGTCC8600.013.02307170
AAGAGCC20550.012.60322870
AACTCCC9500.012.52613870
CACACGG5206.002665E-1112.11521370
CCCCCGC2250.006695458710.8911231
CGGGGGT3451.4230947E-410.14478443
ATCGGGG8750.09.99985870
GGGGGGT4251.0662372E-59.88221350
CACTAGC61700.09.41639970
ACTAGCG4251.149856E-49.05869670
TCGGGGG5452.2409222E-68.99069942
CGGAAGC18300.08.98894470
CCTGGAC4006.2767725E-48.74997764
TAGCTTA46300.08.31521770
CCTCGGA22650.08.1897313
ACTAGCT54950.08.1527570
CGGAACC12150.08.06572916