FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n01_LHY1_c24_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n01_LHY1_c24_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length76
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAAATATTTATTAAATATATTAAGATCAGACTGTATACTACGAA13.4482758620689653No Hit
GAAAGACGTTAATCATTTTTTCCTCCCGTTTTAAAACTAGAAATATCCAA13.4482758620689653No Hit
TTCAAACCCACCGAATGAAATAGAATATCTAAACAACTTTACCGATATTT13.4482758620689653No Hit
AAAAATATGTATATATCTAAACATATTTGAAATTATGATTTAATTATTTT13.4482758620689653No Hit
TCATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGG13.4482758620689653No Hit
GTTTAAAAAAAATATACTTTAAACGTTTATATCATTTAAATTCTAATTAA13.4482758620689653No Hit
TAAGAAATAAAATTTTATTTTCTAAGCAGTTTTGATTTATTTTTTAAAGT13.4482758620689653No Hit
AACTAGTTACAACTAGATGAAGTAATCAATAATTAGGAACATATATCAAT13.4482758620689653No Hit
GTTTGCATCGCATGCGATATTTGGTTCATCAGCTCTTCCCAACAGACGAC13.4482758620689653No Hit
CATTGAGATTTAATCTCGTTTAAATATAAAATCTAAAACTGTTTATAATT13.4482758620689653No Hit
AAAATGATATTATTGCACGGAAAAAAATTATGTGATCCGCAATAATTAAT13.4482758620689653No Hit
GTTATTTATTATGAATCTAAACTCATTATTTATAATAACATAAAAATTGC13.4482758620689653No Hit
AAATAAACTATTATAAGAATTAAGGCATTAGAAATATATTTAGATTAAAT13.4482758620689653No Hit
GCCTTGCACCGAGATAGTGCTTTTTCAACTATCTCAAAATGGAATCTATT13.4482758620689653No Hit
TAGTGTAGAGGTCGATAAATGAGCAAAATATAGTAAAATTGTTATAGAAA13.4482758620689653No Hit
ATTTGTCCGCATTACAAAATATTCATTTATCTTACGAAGAATATCATAAT13.4482758620689653No Hit
TCTCGTCATGCAGCAAAACAATATTAAAAAAGAGATTATTCTAGTAATTA13.4482758620689653No Hit
TCCTTAATTCTGTGAATATATTTATTGGTGGACGAAAAAAAGGTTTCTTG13.4482758620689653No Hit
TTTAACCTTTAGATCATGATAGATACAAATATAATCAACAGATTTTAGGT13.4482758620689653No Hit
GTTAAGTTAGAATTTTTAATATTAAATCGTTTTTACTGTTCTTTAGTAGA13.4482758620689653No Hit
TCCTATATAACTCTTTCCTTGAGCTGATACCATACTCTTCACACCTTTAC13.4482758620689653No Hit
CCATATAATATACAAAAAGAAACTCCAGATATTTGATATCTTTCTCTTTA13.4482758620689653No Hit
TGCTTCGTCATTAAAACTTTCCCTTTGCGATACAAGGATATCAAATGGGG13.4482758620689653No Hit
ATTTCCGCGTTTTGTAATTCACATTATATTTTTAGAATTACCCTCCGCTT13.4482758620689653No Hit
CCCAAAGAATGTAACGGGTTACACGAAATTCTTGCCAATACAATTGTCCA13.4482758620689653No Hit
AAATTTGTTAAAAATTAATAAATTAATACTAGGTTTTACCCGATTTTAGA13.4482758620689653No Hit
GGTAGTTCTATTTTCCTTAGACAACAAAAATAGACGTAGTCGAAATGTGC13.4482758620689653No Hit
GACTATGATAATATGCAACTTTGATAATACTTTGTAAAATTACAAATCCT13.4482758620689653No Hit
GTATTCGATTGTATGGAGTGTTATCTAAATTATAGACATCTACATTAATC13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)