FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12023138
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG744400.6191395291312468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG26550.014.4964911
TATACGG20150.013.5756955
GTATATA64300.013.0148251
CCTACAC11750.012.278573
TATACAG37200.012.0672855
GTACTAG18600.011.9599281
TAGACTG14950.011.72927955
GTATAGA28000.011.7280521
TAGGTAG13400.011.5157015
CTAATAC31800.011.5082183
TCCGCAC11150.011.29179364
TCTAGAC15150.010.9165923
TATACTG35700.010.9042875
GTGTAAG16150.010.7133291
TATTAGG27650.010.7059892
TAATACT49050.010.6630184
TAGTACT21850.010.6029184
CGCACCT12550.010.59690366
TAGACAG20600.010.5552125
ATACGGT21000.010.4958256
Produced by FastQC (version 0.11.5)