FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_Gbc_c83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_Gbc_c83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789277
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC13900.018.3811683
TCCGCAC4500.016.3332264
GTATAGA13350.015.4727741
CGCACCT5200.014.8075966
TCTAGAC7400.014.6620623
TAGGTAG7300.014.3829465
TATACAG20900.013.731475
GTATTAG12250.013.7183711
ACTATGC13650.013.5891638
CCGCACC5450.013.48614565
CGCGTGT2405.571274E-513.12443870
GTCTAAG8400.012.9205151
TATATAG23650.012.7267295
CTAGACT7050.012.4108164
GTATATG20950.012.3664511
TATTAGG13900.012.3387082
GCCGCGT6600.012.19644725
ACCGCGC2305.7206687E-412.1733928
ACGCGTG2601.1776063E-412.11530169
GTATATA35850.012.1096041
Produced by FastQC (version 0.11.5)