FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_Gbc_c77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_Gbc_c77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846070
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG5100.016.47051870
ACCGCGC2602.771958E-816.1529378
GTATTAG15850.015.2410861
TATACAG24850.014.3653895
GTCCTAG5300.013.8720131
TATACGG14300.013.4607815
GTATATA39200.013.2181821
CATATAC25550.013.0131363
TCTATAC18900.012.9624023
ATACGGT14650.012.9002986
TACACCG7750.012.644455
ACGGTGT12300.012.2350848
TAGGTAG8700.012.0682875
TATATAC47600.012.0583023
TATACTG26000.011.9800955
CTATACA18250.011.8897424
CGCGTGT4802.7121132E-911.66661670
TAATACT28500.011.666014
TACGGTG12300.011.666017
CACCGCG4257.4001946E-811.52891323