FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_Gbc_c74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_Gbc_c74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4923650
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG11400.018.11369970
CTAGGAC8000.015.3125273
TAGGTAG8600.014.2437775
GTATTAG17000.014.2105251
ACTCCGC9350.013.475558
CGCGTGT8850.013.44635169
TCTTACG36150.013.16711470
TATACAG23050.013.0581935
CTATACT19350.012.8420084
CTTACGC24400.012.47927770
ACTATGC18700.012.1654268
GTATATA36900.012.144891
TACGCGT17600.012.13045670
CTAATAC22050.012.0635133
TATATAC40400.012.04213
CTAGACT8200.011.9508764
GCGCACT8250.011.87989411
TAACCCG4752.3028406E-911.7891365
TCTAGAC13150.011.7110473
TCCGCAC9050.011.60211364