FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_Gbc_c71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_Gbc_c71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688381
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4650.023.332045
TCCGCAC3350.018.80659964
TAGTCAG5000.018.2022259
TTAGGTA7750.018.0635134
GGTAGTC5700.017.806037
CGCACCT3350.017.76178766
CCGCACC3600.017.50058665
GTATTAG9500.017.3214991
ATAGGAG5700.017.19202833
CAGTGCG1906.1180635E-616.5809759
ACCGCGC1300.00229224416.152958
CTATGCA8100.015.989619
ACTATGC9750.015.7939978
TAGGAGT6250.015.679131534
ACATAGG6050.015.61896931
GGCGTCG2251.7650837E-615.55469332
GGTAGGA5700.015.35184569
TACGGTG6500.015.0760877
ACGGTGT6800.014.9256438
GTAGTCA7600.014.7360268