FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_Gbc_c69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_Gbc_c69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200212
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1553.509558E-822.5803493
ACGCGTG1107.478041E-419.0906670
CGCACCT2104.271169E-818.33385766
CGACGGG1150.001008423618.2598749
TCCGCAC2508.6583896E-1018.2005264
CTAATAC5800.016.896333
GTATTAG5600.016.2538491
ACAGTCC2251.7640341E-615.5547058
TATACAG7550.014.8336265
TATACTG9150.014.5347225
CCGCACC2656.027676E-714.52871765
GCCCGCC1450.004733924314.48558511
TAGACAG3403.25781E-914.4109755
TCATATA10000.014.350412
TATATAG9400.014.1481625
GTATAGG3252.786146E-814.0033171
CATATAC9300.013.9245493
TACGGTG4303.274181E-1113.8364517
CTATACT5350.013.7375654
CTGATCG2556.447106E-613.728179