FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_Ap3-H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_Ap3-H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433772
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1055.468696E-419.9993325
GCGCTCG1651.5942242E-619.09027133
CGCGGCC1150.001007465318.26280813
GTGCGTG5500.017.81758732
TCCGCAC2651.882654E-917.16983864
GGCGCCG1653.396594E-516.96972325
ACGGTGT4750.016.9468048
CGGCCGG1906.117709E-616.58070815
GCTTAGG3005.5479177E-1016.33278870
TCCCGGT1755.6173158E-516.00169812
GTATATA24950.015.9970291
TGCGTAC6850.015.32795651
TATACGG8450.014.9107445
GTAGACT2352.7731403E-614.893124
ACCGTGC1656.5231737E-414.8479898
GCGTACT6650.014.73634952
ATACGGT6900.014.7096556
AGTGCGT7150.014.68482431
TGTAGAC4600.014.4565443
CTGATCG1708.170275E-414.4132959