FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_Ap1-E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_Ap1-E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563786
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCG1000.00969323817.4995787
ACCTCGC1401.8665651E-417.4995778
TACACCG2903.4197E-1016.8961415
TAGGTAG3207.8216544E-1116.4058535
CTTACAC4350.016.0920773
ACAGTCC3401.9645086E-1015.4408048
CTTAGGG1400.00375453315.0001173
CTAGGGG1400.00375453315.0001173
GTATATA18850.014.8585031
TGTCCGA1656.518446E-414.84954910
CCTAGCG2151.9767953E-514.65080730
GTATTAG6650.014.2147271
ATCGCGG1500.005937451513.99966149
CGCTCGT2556.4626147E-613.72559662
CGGGCGG2052.1635772E-413.65820652
GTATAGG5400.013.6151341
GTCTTAC4901.8189894E-1213.5754411
CAGGGCG1550.00737716313.54805854
GCCGTCC1550.00737716313.54805858
ACTGCCC2354.5689398E-513.4039318
Produced by FastQC (version 0.11.5)