FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3.F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3.F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339989
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCG350.001253070240.00941510
AGGATCG350.001254884839.9976465
TGTAGAT3250.039.84380770
GAGCCCC400.002414255435.0082411
ATCGCCG501.7055929E-435.0082412
TATACGG400.002417742734.997945
CCGATGC450.00430547531.1092828
TTAAGGT853.95003E-628.8218334
ATCCTAA755.5510005E-528.0189571
CCCCTAC500.00717957228.0189571
TATCCGC500.007195065328.00659210
TAGCTAG755.578906E-527.998355
GTGTAGA5900.026.1001670
CCTTCTA851.3003811E-424.7226091
CTGCGGA1001.3924549E-524.5057689
ACCATGC1001.3956338E-524.498568
ATACGGA1001.3956338E-524.4985630
GTAGATC1652.6466296E-923.3319670
TATAGTG901.9257549E-423.331965
ACTGGCC750.001831015923.3319598