FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712502
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTGC7550.017.6151948
GTATTAG11650.017.429021
CTACCCC4950.016.969024
CTAGACT5100.016.4699344
CCTTGCG8450.015.7410499
TAGGTAG6700.015.6710195
CGCGGGT2101.5834466E-514.999680553
CTAGACA6750.014.5179414
GGTAGTC6850.014.3060017
CGCGCGG2001.755433E-413.999701554
TCCTTGC16400.013.8713998
TCCTAAG7350.013.8094842
CTCCTCG6850.013.7950727
GTATATG19600.013.7532561
CGGTCTC4851.8189894E-1213.71079518
CCTATAC10600.013.5374463
TCGGTCT5200.013.46100317
GTCCTAA7100.013.3130111
TACCCCT7250.013.0339645
CCCGTCG3801.5561454E-812.89422330
Produced by FastQC (version 0.11.5)