FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829366
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8400.018.7548331
CGCACCT3156.184564E-1116.6668666
AGCGGGG2108.5930697E-716.6659550
GGCGCCG1503.161451E-416.33263225
CCGCACC3301.2551027E-1015.90927865
GGTAGTC5000.015.3993397
CTTACAC4800.015.3130993
CCTAAGC1605.1626645E-415.3130983
GCGGGGA2302.2186778E-615.21673751
TCCGCAC3251.724402E-915.077164
TAGACAG5900.014.8298715
TAGGTAG5300.014.5276785
GTATAGA10250.014.003611
TATACAG14150.013.8509955
CTAGGGT2052.1642378E-413.657954
TAGTCAG4659.094947E-1213.5503979
CGCCGAG1550.007378708613.54780520
TCTATAC9050.013.5364413
TATATAG15300.013.4961525
TATACGG7100.013.3092875
Produced by FastQC (version 0.11.5)