FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887926
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG8050.014.3472515
TAGGTAG4951.8189894E-1213.43380455
GCGAGGC1600.00910131413.12447430
GTATAGA8350.012.9972771
GTATTAG8250.012.7304711
GTATATA24700.012.4727691
GTATATG12150.012.3899451
TCTATAC9650.012.3314393
ACGGTGT6700.012.0144448
CGAAGGC10200.012.00932270
TATACCG7050.011.9144165
ACTGGTC3255.9723698E-611.8456798
TCCTATA7750.011.7420862
TCCGGGG3307.067252E-611.66805311
TACGGTG6600.011.6661997
TACACAG5702.5465852E-1111.6661995
TAAGCAG7350.011.4281135
ACTGTGC3409.867599E-611.3230758
TATACTG12150.011.23411755
CGGGCGG2500.001150924411.19955152