FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292276
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGG604.9846957E-429.16660370
CCGCACC2607.2759576E-1118.8475765
GTATTAG5600.017.502671
CCGCCCG1602.6044041E-517.5026710
ACGGTGT4800.017.4986088
TCCGCAC2852.6739144E-1017.1936164
CGCACCT3104.9112714E-1116.93675666
TACGGTG5100.016.4692787
TATACGG6600.015.90782455
CTAATAC6600.015.9072083
TAGGTAG2906.2300387E-915.6884075
GTATAGA7400.015.610491
TAGGACA2503.121604E-715.3981794
ACAGCGC2302.2182994E-615.2161798
CGCCCGT1901.1326137E-414.73909111
ATACGGT6250.014.5588426
GCACCCG1708.1714214E-414.41284841
CATATAC10900.014.1267693
CACTGGG3504.9039954E-913.9988857
TATACAG11150.013.8105595
Produced by FastQC (version 0.11.5)