FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576138
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7400.016.5573431
TAGACTG3401.9645086E-1015.4408125
CCGCACC2858.5414285E-814.73696265
ACCCTAT3352.6375346E-914.6297691
CCGTGCC1708.1710867E-414.4132549
GTACTAC4750.014.0027791
GTGTAAG4051.8371793E-1013.82990551
CGGCGCC1800.001259858513.6107932
ACCGTGC1800.001259858513.610798
TAGACAG4951.8189894E-1213.4340275
CCTAGGA2354.566236E-513.404792
ACGCCCG2102.650605E-413.3347111
TATACAG10300.013.2521145
GTCTTAG4253.947207E-1013.1790871
TCCGCAC2952.0194002E-613.05095464
TATAGGA6800.012.868162
TAGGACG2456.754344E-512.856844
CTAATAC8450.012.84034253
CGCACCT3002.4384663E-612.83343866
GCTATAC3558.889947E-812.8170063
Produced by FastQC (version 0.11.5)