FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706587
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8950.018.773481
CCGCACC2802.0736479E-1017.50133365
CCTAGGC1250.001763895716.7988193
GCGCACT1350.002945316515.55719611
GTAGGAC2251.7653838E-615.55446153
CGCACCT3401.9645086E-1015.44235366
TCCGCAC3201.382432E-915.31366764
CTACCCC2505.270731E-613.9990164
CTAATAC8500.013.9990163
ACTATGC7300.013.90313158
TCCTTGC7850.013.8206848
CACTCCG1800.001258706913.61254814
GTATAGA8750.013.6018331
TAGGTAG4404.7293724E-1113.5217755
GTATCCT6450.013.0250121
TAGTCAG4304.765752E-1013.024639
GTCCTAA4052.7157512E-912.9647091
TACTATG9000.012.8324317
ACCAGCG3851.8670107E-812.72637757
TATACAG12750.012.6265635
Produced by FastQC (version 0.11.5)