FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-F10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-F10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705673
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAC1000.00968492717.50229510
TCCTTGC11850.016.8346888
CCTAGGA1957.841229E-616.1535952
GCGCACT2201.3938952E-615.91117711
TCCTAAG3803.6379788E-1215.6576522
TACACCG2604.866888E-714.8070295
GACCGCG1450.00474256214.4821097
GGGAGCG1450.00474256214.4821097
CAGGTCG1750.001017292114.0018359
TGTCCGC1750.001017292114.00183510
CACGCGC1500.00593855413.99937221
CGACCCC1550.0073785313.5477843
GTATCCT7250.013.5202031
AGACCGC2901.6678241E-613.2752676
CCTTGCG4601.0004442E-1012.9364789
CCCGGCC2508.149438E-512.60165215
ACGCGCA2254.7491965E-412.44498239
TGCGTGG2254.7527172E-412.4438868
GTATATA22050.012.3836641
GTCCTAA4551.1623342E-912.3103891
Produced by FastQC (version 0.11.5)