FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-F06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-F06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940320
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3153.6379788E-1217.77828866
GGCGCCG1503.1610907E-416.3329625
CGCGCAT1708.175306E-414.4125540
TCCGCAC3504.9039954E-914.00004164
TACGGTG7550.013.9066097
GTATATG13100.013.6284521
GTATTAG10900.013.4887111
TATACGG8100.013.3944115
TGTCCCG1600.009094213.12605310
CCGCACC3751.2911187E-813.06670565
GGGCTCA2952.0184525E-613.05189411
TCTATAC9150.013.0051683
GCCCTAG1900.001890590412.8971011
CCTAGGC1900.001893891512.8941118
AGCCCCC2508.162304E-512.59971155
ACGGTGT7250.012.5511158
GTCCTAA4204.665708E-912.5022931
ATACGGT9250.012.4858796
TATACAG13300.012.367825
GTATATA25350.012.2902211