FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606943
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCC1401.8624066E-417.50473213
CGCACCT2201.3953686E-615.9094366
TATACGG6550.015.4950685
CCTAGCG1450.0047432614.48171530
GGCCCGC1500.005925966414.00378515
TACGTAG3102.3878965E-713.5474125
GTAGGAC1600.00910058713.1244633
CTTACAC4851.8189894E-1112.9891593
CCGCACC2701.1653452E-512.96323965
GTCCTAC2456.735934E-512.8606191
TACGGTG6050.012.7263567
ACGGTGT5800.012.6715018
TAGGACA3601.0682015E-712.6379794
CTACACA5003.092282E-1112.59909254
ATACGGT7250.012.550826
TAATACT13350.012.321214
CCGTCTC2852.0261534E-512.2840219
GTATATA20000.012.2533111
TGCGAGT2601.17396725E-412.1186610
GACTGTA3852.558918E-711.8173317
Produced by FastQC (version 0.11.5)