FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559672
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1052.041877E-523.3323828
TAGGGCG950.00721342518.4203015
CCGCACC2901.8189894E-1118.1038765
CCGCGCA1000.00968342217.5026539
AGCTCGC2954.3655746E-1016.61002560
CTAATAC7750.015.8058073
TAGGTAG4000.015.7493575
CAGCTCG2906.2282197E-915.68951859
TATACCG4950.015.5549215
TCCGCAC3201.3842509E-915.31285864
CGCACCT3201.3842509E-915.31285866
TAATACT13600.014.9258624
GACGTGA3302.1391315E-914.8478833
GTATATA24000.014.4396891
GCGCAGC3655.7298166E-1014.38297656
CGCGCAT1500.005934309714.00077640
CTCGCGT3252.7972419E-813.99987862
GCGACTC2253.0356172E-513.99942934
CGACGTG3758.60382E-1013.99942932
TATACTG9800.013.9280035