FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840694
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3500.018.00122364
CTAGGAC2401.0311851E-817.4992893
GGCGGGC1401.8674156E-417.49881253
CTAATAC8200.017.0724743
GTATTAG8000.016.628391
CGCACCT3800.016.58007466
CCGCACC4050.016.42086865
TAGGTAG4600.015.9771755
TTAGGTA7100.015.2806534
TATTAGG9400.015.2653362
TAATACT14250.014.4902454
ACTATGC10200.014.4107878
GCGCGGC1951.4129556E-414.36150912
CGACCTC3707.039489E-1014.18822651
GTATATA20300.013.9683631
GGCGCGG1550.007368020213.5507810
TATACTG9900.013.433435
ACAGGGC3152.9020885E-713.3324288
TATACAG12350.013.3189335
ACTCCGC3456.127448E-813.187518
Produced by FastQC (version 0.11.5)