FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522225
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6650.018.9462955
TCCGCAC3551.8189894E-1216.76027964
GGTAGTC5900.016.6092287
TCTATAC11800.014.8308453
TAGTCAG5500.014.6393079
GTATTAG10850.013.8756841
GTATAGA12550.013.6699841
CCCTATA5950.013.5297172
TAGACAG8550.013.5080075
GTATATA29650.013.3434891
TACACCG5051.8189894E-1213.167575
GTAGGAG6650.013.1574170
CGACGGG2701.1666687E-512.96248649
TATTAGG10650.012.8171912
CCTATAC7450.012.6848513
ATAGGAC6950.012.5902133
ACATAGG7000.012.49929231
TATACGG9250.012.485785
CTAGGAC4509.804353E-1012.4447263
GTCCTAG3103.509862E-612.4235771
Produced by FastQC (version 0.11.5)