FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406766
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10400.018.8495271
GCGCACT2508.6765795E-1018.20136611
GGACCGC1350.002948423415.5551086
TATACTG13350.015.2055555
TCCGCAC3858.185452E-1114.5456464
CCTAGAC5250.013.3329493
CACCGTG2901.6683389E-613.2754797
CTAGGGC2659.621717E-613.2071663
CCTATAC7350.012.8567723
TACACCG4651.200533E-1012.795335
GTATATA26600.012.7654431
GTCTTAG5800.012.6746821
GTATAGG7750.012.6474251
CATATAC17650.012.49255853
GTATAGA11600.012.3729031
CTAGACC6550.012.2897224
CGCACTC3458.737006E-712.17482812
CCGCACC4601.3860699E-912.17406865
CTAATAC11300.012.0792983
TACTGTG9300.012.0426647
Produced by FastQC (version 0.11.5)