FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423435
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3750.017.73331864
GTATTAG11050.017.1065271
TAGGTAG6100.016.6386435
CCGCACC4550.015.38460265
TATACCG7950.014.9679225
TCCTTGC13700.014.8168948
GACGCGC2604.863505E-714.80859538
CGCACCT4051.0913936E-1114.69134466
GTATCCT9550.014.6617911
CCTATAC8050.014.3475173
TCTATAC11500.014.304043
CGCGCAT2454.271764E-614.28658640
CTATACT11100.014.1885914
TAGTCAG5550.013.8764389
CTTCCTA9450.013.7056711
TATTAGG10500.013.6666552
CTACCCC3102.3887333E-713.5478164
GTATAGA10600.013.53968051
GTCGGGA1850.001549577213.24268444
GGTAGTC6350.013.2277897
Produced by FastQC (version 0.11.5)