FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182737
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3750.018.6667466
CCGCACC3900.017.9487965
TCCGCAC3850.017.27279564
GTATAGA10450.017.0833641
TAGGTAG4700.016.382675
GTATTAG9050.016.2450181
CACCTCG1957.8435005E-616.1535443
GGACCGC1656.5243954E-414.8482036
GTATATA22150.014.5389681
CTAGGAC3202.2828317E-814.2188063
GTGTAGG3453.9999577E-914.204581
CGGGACC2759.1726906E-713.9997344
GTATAGG7150.013.21834851
TAAGACT7150.013.2165334
CTTACAC4406.87578E-1012.7273233
CTAATAC9700.012.6289153
TAGGACT3356.126211E-712.5370764
TACACCG4204.6802597E-912.4997635
ACGGTGT5950.012.3527078
CTTAGAC6300.012.222273
Produced by FastQC (version 0.11.5)